PrimePCR™ Probe Assay: CIZ1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CIZ1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CIZ1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024371
Assay Design:   Exonic
Chromosome Location:   9:130940648-130940751question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000372954 ENST00000277465 ENST00000372948 ENST00000372938 ENST00000415526 ENST00000393608 ENST00000538431 ENST00000357558 ENST00000325721 ENST00000537314 ENST00000541172

Gene Information

The protein encoded by this gene is a zinc finger DNA binding protein that interacts with CIP1 part of a complex with cyclin E. The encoded protein may regulate the cellular localization of CIP1. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2012]

Gene Symbol:   CIZ1
Gene Name:   CDKN1A interacting zinc finger protein 1
Aliases:   LSFR1, NP94, ZNF356
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000148337
Entrez:   25792
Chromosome Mapping:   9q34.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.610000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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