PrimePCR™ Probe Assay: TNP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024164
Assay Design:   Exonic
Chromosome Location:   2:217724650-217724779question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000236979

Gene Information

Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines (see PRM1 MIM 182880; PRM2 MIM 182890) (Luerssen et al. 1990 [PubMed 2249851]).[supplied by OMIM Mar 2008]

Gene Symbol:   TNP1
Gene Name:   transition protein 1 (during histone to protamine replacement)
Aliases:   TP1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000118245
Entrez:   7141
Chromosome Mapping:   2q35-q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.180000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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