PrimePCR™ Probe Assay: NKAIN4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NKAIN4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NKAIN4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023997
Assay Design:   Exonic
Chromosome Location:   20:61879044-61880193question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000370313 ENST00000370316 ENST00000370307 ENST00000370317

Gene Information

NKAIN4 is a member of a family of mammalian proteins (see NKAIN1; MIM 612871) with similarity to Drosophila Nkain and interacts with the beta subunit of NaK-ATPase (ATP1B1; MIM 182330) (Gorokhova et al. 2007 [PubMed 17606467]).[supplied by OMIM Jun 2009]

Gene Symbol:   NKAIN4
Gene Name:   Na+/K+ transporting ATPase interacting 4
Aliases:   C20orf58, FAM77A, bA261N11.2
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000101198
Entrez:   128414
Chromosome Mapping:   20q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.290000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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