PrimePCR™ SYBR® Green Assay: AQP12A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058594

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AQP12A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AQP12A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057489
Assay Design:   exonic
Chromosome Location:   2:241631712-241631894question
Amplicon Length:   153
Splice Variants Targeted:   ENST00000393986 ENST00000328119 ENST00000302516 ENST00000467148 ENST00000505667 ENST00000243903 ENST00000264079 ENST00000431216 ENST00000553001 ENST00000543202 ENST00000440966 ENST00000382549 ENST00000290155 ENST00000458138 ENST00000540881 ENST00000558850 ENST00000559185 ENST00000442396 ENST00000561251 ENST00000559155 ENST00000262236 ENST00000373115 ENST00000264039 ENST00000420138 ENST00000407834 ENST00000337801 ENST00000429564 ENST00000328879 ENST00000302334 ENST00000309156 ENST00000394211 ENST

Gene Information

Description Not Available

Gene Symbol:   AQP12A
Gene Name:   aquaporin 12A
Aliases:   AQP12, AQPX2
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000184945
Entrez:   375318
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.040000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download