PrimePCR™ SYBR® Green Assay: NLRP11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058047

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: NLRP11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NLRP11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056942
Assay Design:   exonic
Chromosome Location:   19:56320703-56320849question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000589824 ENST00000592953 ENST00000589093 ENST00000443188 ENST00000360133

Gene Information

NALPs are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs such as NALP11 have an N-terminal pyrin (MEFV; MIM 608107) domain (PYD) followed by a NACHT domain a NACHT-associated domain (NAD) and a C-terminal leucine-rich repeat (LRR) region. The long NALP NALP1 (MIM 606636) also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NALPs are implicated in the activation of proinflammatory caspases (e.g. CASP1; MIM 147678) via their involvement in multiprotein complexes called inflammasomes (Tschopp et al. 2003 [PubMed 12563287]).[supplied by OMIM Mar 2008]

Gene Symbol:   NLRP11
Gene Name:   NLR family, pyrin domain containing 11
Aliases:   CLR19.6, FLJ26273, NALP11, NOD17, PAN10, PYPAF6, PYPAF7
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000179873
Entrez:   204801
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.240000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download