PrimePCR™ SYBR® Green Assay: HLA-DPB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057973

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HLA-DPB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HLA-DPB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056868
Assay Design:   exonic
Chromosome Location:   6:33054687-33054800question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000433800 ENST00000444931 ENST00000447674 ENST00000552574 ENST00000411749 ENST00000441981 ENST00000411722 ENST00000418931 ENST00000416804 ENST00000454006 ENST00000411424 ENST00000458614 ENST00000548235 ENST00000425130 ENST00000411558 ENST00000455122 ENST00000383102 ENST00000441857 ENST00000434121 ENST00000548608 ENST00000551599 ENST00000282466 ENST00000415996 ENST00000447783 ENST00000445925 ENST00000548074 ENST00000548907 ENST00000432661 ENST00000399491 ENST00000399500

Gene Information

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities resulting in up to 4 different molecules. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DPB1
Gene Name:   major histocompatibility complex, class II, DP beta 1
Aliases:   DPB1, HLA-DP1B, HLA-DPB
RefSeq:   NT_113891.2 NC_000006.11 NT_007592.15 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000223865
Entrez:   3115
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download