PrimePCR™ SYBR® Green Assay: ZIC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057888

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZIC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZIC4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056783
Assay Design:   exonic
Chromosome Location:   3:147108855-147108992question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000267294 ENST00000383075 ENST00000484399 ENST00000473123 ENST00000491672 ENST00000425731 ENST00000525172 ENST00000488404 ENST00000282928 ENST00000287538 ENST00000370606

Gene Information

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1 a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq Dec 2009]

Gene Symbol:   ZIC4
Gene Name:   Zic family member 4
Aliases:   FLJ42609, FLJ45833
RefSeq:   NC_000003.11 NT_005612.16 NG_015886.1 NG_009242.1
Ensembl:   ENSG00000174963
Entrez:   84107
Chromosome Mapping:   3q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 39.160000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download