PrimePCR™ SYBR® Green Assay: SLC25A10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057864

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC25A10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056759
Assay Design:   exonic
Chromosome Location:   17:79687871-79688015question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000309100 ENST00000331531 ENST00000350690 ENST00000375401 ENST00000404049 ENST00000375379 ENST00000453386 ENST00000382191 ENST00000538780 ENST00000358906 ENST00000520539 ENST00000220959 ENST00000521922 ENST00000602020 ENST00000594714 ENST00000595211

Gene Information

The dicarboxylate carrier catalyzes the transport of dicarboxylates such as malate and succinate across the mitochondrial membrane in exchange for phosphate sulfate and thiosulfate thus supplying substrates for the Krebs cycle gluconeogenesis urea synthesis and sulfur metabolism.[supplied by OMIM Jul 2002]

Gene Symbol:   SLC25A10
Gene Name:   solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
Aliases:   DIC
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000183048
Entrez:   1468
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.250000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download