PrimePCR™ SYBR® Green Assay: PLEKHJ1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057846

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056741
Assay Design:   exonic
Chromosome Location:   19:2233251-2233350question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000257821 ENST00000511828 ENST00000290607 ENST00000343070 ENST00000307331 ENST00000568085 ENST00000568923 ENST00000592552 ENST00000589097 ENST00000326631 ENST00000373277 ENST00000420366 ENST00000314830 ENST00000373276 ENST00000373274 ENST00000429553 ENST00000258341 ENST00000300482 ENST00000397928

Gene Information

Description Not Available

Gene Symbol:   PLEKHJ1
Gene Name:   pleckstrin homology domain containing, family J member 1
Aliases:   FLJ10297, GNRPX
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000104886
Entrez:   55111
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.890000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download