PrimePCR™ SYBR® Green Assay: HAGHL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0057845

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HAGHL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HAGHL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056740
Assay Design:   exonic
Chromosome Location:   16:779473-779564question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000413998 ENST00000372470 ENST00000568579 ENST00000567758 ENST00000424767 ENST00000545376 ENST00000254977 ENST00000159111 ENST00000536461 ENST00000371030 ENST00000225296 ENST00000382745 ENST00000262318 ENST00000448525 ENST00000403662 ENST00000406230 ENST00000262825 ENST00000536485 ENST00000549114 ENST00000341413 ENST00000564537 ENST00000389703

Gene Information

Description Not Available

Gene Symbol:   HAGHL
Gene Name:   hydroxyacylglutathione hydrolase-like
Aliases:   MGC2605
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103253
Entrez:   84264
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download