PrimePCR™ SYBR® Green Assay: UNC13D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057843

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056738
Assay Design:   exonic
Chromosome Location:   17:73832895-73832985question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000389840 ENST00000585328 ENST00000257555 ENST00000535125 ENST00000541395 ENST00000576183 ENST00000207549 ENST00000412096

Gene Information

This gene encodes a protein that is a member of the UNC13 family containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3 a genetically heterogeneous rare autosomal recessive disorder. [provided by RefSeq Jul 2008]

Gene Symbol:   UNC13D
Gene Name:   unc-13 homolog D (C. elegans)
Aliases:   FHL3, HLH3, HPLH3, Munc13-4
RefSeq:   NC_000017.10 NG_007266.1 NT_010783.15
Ensembl:   ENSG00000092929
Entrez:   201294
UniGene:   Hs.41045
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.410000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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