PrimePCR™ SYBR® Green Assay: STXBP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057841

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: STXBP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: STXBP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056736
Assay Design:   exonic
Chromosome Location:   19:7707135-7707321question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000373887 ENST00000540010 ENST00000546084 ENST00000441779 ENST00000221283 ENST00000414284

Gene Information

This gene encodes a member of the STXBP/unc-18/SEC1 family. These genes are conserved across species from yeast to man and are believed to be involved in protein trafficking from the golgi apparatus to the plasma membrane. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Mar 2010]

Gene Symbol:   STXBP2
Gene Name:   syntaxin binding protein 2
Aliases:   FHL5, Hunc18b, MUNC18-2, UNC18-2, UNC18B, pp10122
RefSeq:   NC_000019.9 NT_077812.2 NG_016709.1
Ensembl:   ENSG00000076944
Entrez:   6813
Chromosome Mapping:   19p13.3-p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.790000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download