PrimePCR™ SYBR® Green Assay: DNASE1L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057836

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056731
Assay Design:   exonic
Chromosome Location:   X:153631061-153631290question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000333244 ENST00000447614 ENST00000359470 ENST00000393638 ENST00000369809 ENST00000447892 ENST00000309585 ENST00000014935 ENST00000369808 ENST00000369807 ENST00000601862 ENST00000597132 ENST00000598641 ENST00000595836 ENST00000598223 ENST00000596762 ENST00000598514

Gene Information

This gene encodes a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants encoding the same protein have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   DNASE1L1
Gene Name:   deoxyribonuclease I-like 1
Aliases:   DNAS1L1, DNASEX, DNL1L, XIB
RefSeq:   NC_000023.10 NG_009634.1 NG_012884.1 NG_012890.1 NT_167198.1
Ensembl:   ENSG00000013563
Entrez:   1774
UniGene:   Hs.401929
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.140000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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