PrimePCR™ SYBR® Green Assay: TRA2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057696

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056591
Assay Design:   exonic
Chromosome Location:   3:185639899-185641673question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000453386 ENST00000259043 ENST00000382191

Gene Information

This gene encodes a nuclear protein which functions as sequence-specific serine/arginine splicing factor which plays a role in mRNA processing splicing patterns and gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq Aug 2011]

Gene Symbol:   TRA2B
Gene Name:   transformer 2 beta homolog (Drosophila)
Aliases:   DKFZp686F18120, Htra2-beta, SFRS10, SRFS10, TRA2-BETA, TRAN2B
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000136527
Entrez:   6434
UniGene:   Hs.533122
Chromosome Mapping:   3q26.2-q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.590000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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