PrimePCR™ SYBR® Green Assay: DLG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057649

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DLG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DLG1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056544
Assay Design:   exonic
Chromosome Location:   3:196807983-196812549question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000314062 ENST00000357674 ENST00000392382 ENST00000450955 ENST00000443183 ENST00000448528 ENST00000422288 ENST00000452595 ENST00000419354 ENST00000346964

Gene Information

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation signal transduction cell proliferation synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene but the full-length nature of some of the variants is not known. [provided by RefSeq Feb 2011]

Gene Symbol:   DLG1
Gene Name:   discs, large homolog 1 (Drosophila)
Aliases:   DKFZp761P0818, DKFZp781B0426, DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
RefSeq:   NC_000003.11 NT_029928.13
Ensembl:   ENSG00000075711
Entrez:   1739
Chromosome Mapping:   3q29

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.070000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download