PrimePCR™ SYBR® Green Assay: ATP6V1D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057647

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056542
Assay Design:   exonic
Chromosome Location:   14:67804967-67805116question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000555474 ENST00000216442 ENST00000555431

Gene Information

This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c" and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP6V1D
Gene Name:   ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
Aliases:   ATP6M, VATD, VMA8
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100554
Entrez:   51382
UniGene:   Hs.272630
Chromosome Mapping:   14q23-q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 36.390000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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