PrimePCR™ SYBR® Green Assay: SUPT5H, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057558

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SUPT5H, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SUPT5H, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056453
Assay Design:   exonic
Chromosome Location:   19:39955687-39957209question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000173229 ENST00000538852 ENST00000546090 ENST00000599117 ENST00000359191 ENST00000598725 ENST00000593727 ENST00000432763 ENST00000402194 ENST00000331715 ENST00000373580 ENST00000310127 ENST00000525591 ENST00000539485 ENST00000354233 ENST00000409681 ENST00000263857 ENST00000599201

Gene Information

Description Not Available

Gene Symbol:   SUPT5H
Gene Name:   suppressor of Ty 5 homolog (S. cerevisiae)
Aliases:   FLJ34157, SPT5, SPT5H, Tat-CT1
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000196235
Entrez:   6829
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.530000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download