PrimePCR™ SYBR® Green Assay: METTL23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057553

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: METTL23, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: METTL23, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056448
Assay Design:   exonic
Chromosome Location:   17:74725865-74729271question
Amplicon Length:   194
Splice Variants Targeted:   ENST00000409548 ENST00000550983 ENST00000357796 ENST00000599568 ENST00000315684 ENST00000284311 ENST00000524490 ENST00000524767 ENST00000525919 ENST00000525038 ENST00000529032 ENST00000334736 ENST00000343878 ENST00000333861 ENST00000333640 ENST00000443375 ENST00000329540 ENST00000304758 ENST00000371962 ENST00000524145 ENST00000371959 ENST00000357260 ENST00000261637 ENST00000341690 ENST00000567759 ENST00000566732 ENST00000378541 ENST00000541676 ENST00000316157 ENST00000382496 ENST00000418100 ENST

Gene Information

Description Not Available

Gene Symbol:   METTL23
Gene Name:   methyltransferase like 23
Aliases:   C17orf95
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000181038
Entrez:   124512
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.260000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download