PrimePCR™ SYBR® Green Assay: ARNTL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057532

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARNTL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARNTL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056427
Assay Design:   exonic
Chromosome Location:   11:13347996-13371587question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000394001 ENST00000571312 ENST00000576522 ENST00000534544 ENST00000527998 ENST00000533520 ENST00000389707 ENST00000401424 ENST00000403290 ENST00000403510 ENST00000482049 ENST00000531665 ENST00000396441 ENST00000361003 ENST00000389708 ENST00000373189 ENST00000409890 ENST00000315658 ENST00000393852 ENST00000409344 ENST00000409013

Gene Information

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ARNTL
Gene Name:   aryl hydrocarbon receptor nuclear translocator-like
Aliases:   BMAL1, BMAL1c, JAP3, MGC47515, MOP3, PASD3, TIC, bHLHe5
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000133794
Entrez:   406
Chromosome Mapping:   11p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.330000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download