PrimePCR™ SYBR® Green Assay: AGBL5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057516

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AGBL5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AGBL5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056411
Assay Design:   exonic
Chromosome Location:   2:27275951-27276272question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000333478 ENST00000421160 ENST00000541401 ENST00000409195 ENST00000409273 ENST00000295237 ENST00000421915 ENST00000453161 ENST00000451003 ENST00000323064 ENST00000360131 ENST00000519540 ENST00000342992 ENST00000589042 ENST00000591111 ENST00000434704 ENST00000439476 ENST00000525528 ENST00000395986 ENST00000533131 ENST00000356660 ENST00000418212 ENST00000533246 ENST00000530861 ENST00000395983 ENST00000438929 ENST00000532997 ENST00000395981 ENST00000395978 ENST00000525950 ENST00000314915 ENST

Gene Information

Description Not Available

Gene Symbol:   AGBL5
Gene Name:   ATP/GTP binding protein-like 5
Aliases:   FLJ21839
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000084693
Entrez:   60509
Chromosome Mapping:   2p23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.330000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download