PrimePCR™ SYBR® Green Assay: SLC25A39, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057509

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A39, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC25A39, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056404
Assay Design:   exonic
Chromosome Location:   17:42398854-42399094question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000225308 ENST00000377095 ENST00000590194 ENST00000586016 ENST00000537904 ENST00000588767 ENST00000588049 ENST00000585636 ENST00000373218

Gene Information

SLC25A39 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al. 2006 [PubMed 16949250]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC25A39
Gene Name:   solute carrier family 25, member 39
Aliases:   CGI69, FLJ22407
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000013306
Entrez:   51629
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.990000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download