PrimePCR™ SYBR® Green Assay: EIF4EBP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control; Same primer pair as used in probe assay qHsaCEP0056044

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EIF4EBP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EIF4EBP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048784
Assay Design:   exonic
Chromosome Location:   5:139927261-139927350question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000310331 ENST00000544915 ENST00000266503

Gene Information

This gene encodes a member of the EIF4EBP family which consists of proteins that bind to eukaryotic translation initiation factor 4E and regulate its assembly into EIF4F the multi-subunit translation initiation factor that recognizes the mRNA cap structure. Read-through transcription from the neighboring upstream gene (MASK or ANKHD1) generates a transcript (MASK-BP3) that encodes a protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq Oct 2010]

Gene Symbol:   EIF4EBP3
Gene Name:   eukaryotic translation initiation factor 4E binding protein 3
Aliases:   4E-BP3, 4EBP3
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000243056
Entrez:   8637
Chromosome Mapping:   5q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.520000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download