PrimePCR™ SYBR® Green Assay: ANKRD63, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qHsaCEP0055974

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANKRD63, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANKRD63, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048714
Assay Design:   exonic
Chromosome Location:   15:40574618-40574714question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000389840 ENST00000585328 ENST00000373736 ENST00000530145 ENST00000434396 ENST00000411589 ENST00000354064 ENST00000313236 ENST00000524725 ENST00000359862 ENST00000395955 ENST00000539778 ENST00000301459 ENST00000597742 ENST00000596699 ENST00000599473 ENST00000598413 ENST00000598958

Gene Information

Description Not Available

Gene Symbol:   ANKRD63
Gene Name:   ankyrin repeat domain 63
Aliases:   Not Available
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000230778
Entrez:   100131244
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.310000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download