PrimePCR™ SYBR® Green Assay: TAS2R46, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055955

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TAS2R46, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TAS2R46, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048695
Assay Design:   exonic
Chromosome Location:   12:11214060-11214183question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000573885 ENST00000571012 ENST00000533467 ENST00000539585 ENST00000574263 ENST00000576640

Gene Information

TAS2R46 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al. 2002 [PubMed 12584440]). For further information on TAS2Rs see MIM 604791.[supplied by OMIM Sep 2009]

Gene Symbol:   TAS2R46
Gene Name:   taste receptor, type 2, member 46
Aliases:   MGC134865, T2R46, T2R54
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000226761
Entrez:   259292
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.060000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download