PrimePCR™ SYBR® Green Assay: PHB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055894

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PHB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PHB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048634
Assay Design:   exonic
Chromosome Location:   12:7077615-7077727question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000535923 ENST00000542912 ENST00000545167 ENST00000536316 ENST00000544134 ENST00000399433 ENST00000440277 ENST00000600594 ENST00000599074 ENST00000598395 ENST00000596553 ENST00000594598 ENST00000597988 ENST00000601193

Gene Information

Description Not Available

Gene Symbol:   PHB2
Gene Name:   prohibitin 2
Aliases:   BAP, BCAP37, Bap37, MGC117268, PNAS-141, REA, p22
RefSeq:   NC_000012.11 NG_021408.1 NT_009759.16
Ensembl:   ENSG00000215021
Entrez:   11331
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.140000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download