PrimePCR™ SYBR® Green Assay: MT1H, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0055767

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MT1H, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MT1H, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048507
Assay Design:   exonic
Chromosome Location:   16:56703751-56704442question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000544264 ENST00000164024 ENST00000252971 ENST00000530544 ENST00000402868 ENST00000330479 ENST00000402034 ENST00000546530 ENST00000261729 ENST00000446861 ENST00000548055 ENST00000358743 ENST00000347557 ENST00000404574 ENST00000296473 ENST00000417270 ENST00000530438 ENST00000327111 ENST00000239462 ENST00000542466 ENST00000370060 ENST00000370055 ENST00000361699 ENST00000543994 ENST00000370057 ENST00000538883 ENST00000361981 ENST00000333206 ENST00000420878 ENST00000310677 ENST00000554068 ENST

Gene Information

Description Not Available

Gene Symbol:   MT1H
Gene Name:   metallothionein 1H
Aliases:   MGC70702, MT1
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000205358
Entrez:   4496
Chromosome Mapping:   16q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download