PrimePCR™ SYBR® Green Assay: ZNF155, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055732

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ZNF155, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF155, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048472
Assay Design:   exonic
Chromosome Location:   19:44495700-44495817question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000381577 ENST00000381573 ENST00000269973 ENST00000589703 ENST00000588140 ENST00000591168 ENST00000587682 ENST00000251269 ENST00000592350 ENST00000590411 ENST00000591532 ENST00000407951 ENST00000270014 ENST00000590615 ENST00000586454 ENST00000429154 ENST00000585632 ENST00000585491 ENST00000587846 ENST00000187879 ENST00000391960 ENST00000270645 ENST00000434772 ENST00000585552 ENST00000591850 ENST00000421176 ENST00000589680 ENST00000336976 ENST00000589870 ENST00000591511 ENST00000589155 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF155
Gene Name:   zinc finger protein 155
Aliases:   MGC161655, pHZ-96
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000204920
Entrez:   7711
Chromosome Mapping:   19q13.2-q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download