PrimePCR™ SYBR® Green Assay: CARD16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055711

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CARD16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CARD16, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048451
Assay Design:   exonic
Chromosome Location:   11:104912424-104915311question
Amplicon Length:   186
Splice Variants Targeted:   ENST00000359866 ENST00000373257 ENST00000332312 ENST00000527979 ENST00000533400 ENST00000436863 ENST00000525825 ENST00000528974 ENST00000598974 ENST00000593315 ENST00000393136 ENST00000375706 ENST00000375704 ENST00000525374 ENST00000528513 ENST00000375707 ENST00000311069 ENST00000373048 ENST00000330210 ENST00000427468 ENST00000540011 ENST00000400677

Gene Information

Description Not Available

Gene Symbol:   CARD16
Gene Name:   caspase recruitment domain family, member 16
Aliases:   COP, COP1, PSEUDO-ICE
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000204397
Entrez:   114769
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.230000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download