PrimePCR™ SYBR® Green Assay: ZNF248, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055538

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF248, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF248, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048278
Assay Design:   exonic
Chromosome Location:   10:38126953-38145234question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000576305 ENST00000571382 ENST00000570495 ENST00000563169 ENST00000564323 ENST00000562156 ENST00000573685 ENST00000573113 ENST00000398253 ENST00000292928 ENST00000439428 ENST00000435416 ENST00000395867 ENST00000374648 ENST00000395873 ENST00000395874 ENST00000357328 ENST00000570010 ENST00000464850 ENST00000332307 ENST00000393430 ENST00000567481

Gene Information

Description Not Available

Gene Symbol:   ZNF248
Gene Name:   zinc finger protein 248
Aliases:   KIAA0137, MGC131882, bA162G10.3
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000198105
Entrez:   57209
Chromosome Mapping:   10p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download