PrimePCR™ SYBR® Green Assay: HSPB9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055485

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HSPB9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HSPB9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048225
Assay Design:   exonic
Chromosome Location:   17:40275005-40275094question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000372172 ENST00000372168 ENST00000585732 ENST00000585708 ENST00000586184 ENST00000588077 ENST00000587995 ENST00000382722 ENST00000331243 ENST00000295092 ENST00000381848 ENST00000355067 ENST00000268876 ENST00000394570 ENST00000591048 ENST00000433649 ENST00000378449 ENST00000602795 ENST00000344018 ENST00000299441 ENST00000238936 ENST00000396801 ENST00000355772 ENST00000396799 ENST00000417772 ENST00000376170 ENST00000376142 ENST00000359188 ENST00000376139 ENST00000376160 ENST00000376166 ENST

Gene Information

Description Not Available

Gene Symbol:   HSPB9
Gene Name:   heat shock protein, alpha-crystallin-related, B9
Aliases:   CT51, FLJ27437
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000197723
Entrez:   94086
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download