PrimePCR™ SYBR® Green Assay: TBC1D28, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055294

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TBC1D28, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBC1D28, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048034
Assay Design:   exonic
Chromosome Location:   17:18545149-18545243question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000341846 ENST00000372991 ENST00000511642 ENST00000372987 ENST00000415497 ENST00000372988 ENST00000414200 ENST00000584301 ENST00000580596 ENST00000464963 ENST00000578506 ENST00000345096 ENST00000575220 ENST00000572213 ENST00000573652 ENST00000575211 ENST00000405044

Gene Information

Description Not Available

Gene Symbol:   TBC1D28
Gene Name:   TBC1 domain family, member 28
Aliases:   FLJ40244, MGC138217, MGC138221
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000189375
Entrez:   254272
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.240000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download