This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0055159
PrimePCR™ PreAmp for SYBR® Green Assay: FNBP1, Human
PrimePCR™ Template for SYBR® Green Assay: FNBP1, Human
The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain a proline-rich motif a second coiled-coil domain a Rho family protein-binding domain (RBD) and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2) tankyrase (TNKS) and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.