PrimePCR™ SYBR® Green Assay: ESPN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055142

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ESPN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ESPN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047882
Assay Design:   exonic
Chromosome Location:   1:6517314-6520130question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000529997 ENST00000377828 ENST00000461727 ENST00000434576 ENST00000416731 ENST00000303965 ENST00000359246 ENST00000375376

Gene Information

This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization dimensions dynamics and signaling capacities of the actin filament-rich microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq Nov 2009]

Gene Symbol:   ESPN
Gene Name:   espin
Aliases:   DFNB36, DKFZp434A196, DKFZp434G2126
RefSeq:   NC_000001.10 NG_015866.1 NT_021937.19
Ensembl:   ENSG00000187017
Entrez:   83715
Chromosome Mapping:   1p36.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.950000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download