PrimePCR™ SYBR® Green Assay: HYAL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055121

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HYAL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HYAL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047861
Assay Design:   exonic
Chromosome Location:   3:50330533-50330625question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000576798 ENST00000571109 ENST00000572961 ENST00000572211 ENST00000359051 ENST00000336307 ENST00000415204 ENST00000513170 ENST00000450982 ENST00000418929 ENST00000261917 ENST00000462833 ENST00000263674 ENST00000401399 ENST00000367172 ENST00000367171 ENST00000367170 ENST00000338515 ENST00000339876 ENST00000338586 ENST00000539706 ENST00000360049 ENST00000367169 ENST00000543973

Gene Information

This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related) and some transcripts of each gene share a portion of the first exon. [provided by RefSeq Jan 2011]

Gene Symbol:   HYAL3
Gene Name:   hyaluronoglucosaminidase 3
Aliases:   HYAL-3, LUCA-3, LUCA3
RefSeq:   NG_009295.1 NC_000003.11 NT_022517.18
Ensembl:   ENSG00000186792
Entrez:   8372
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.570000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download