PrimePCR™ SYBR® Green Assay: SPATC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055106

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SPATC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPATC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047846
Assay Design:   exonic
Chromosome Location:   8:145086688-145086829question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000530478 ENST00000562950 ENST00000567097 ENST00000569368 ENST00000377470 ENST00000447830 ENST00000561623 ENST00000356183 ENST00000381873 ENST00000381867 ENST00000526406 ENST00000533063 ENST00000262580 ENST00000525208 ENST00000409974 ENST00000252998 ENST00000245105 ENST00000539824 ENST00000432012

Gene Information

Description Not Available

Gene Symbol:   SPATC1
Gene Name:   spermatogenesis and centriole associated 1
Aliases:   MGC61633, SPATA15
RefSeq:   NC_000008.10 NT_008046.16 NW_003315923.1
Ensembl:   ENSG00000186583
Entrez:   375686
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.730000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download