PrimePCR™ SYBR® Green Assay: ZNF529, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055053

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ZNF529, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF529, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047793
Assay Design:   exonic
Chromosome Location:   19:37045606-37047022question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000343338 ENST00000532539 ENST00000525502 ENST00000534569 ENST00000427810 ENST00000529678 ENST00000533036 ENST00000528410 ENST00000438262 ENST00000526601 ENST00000005082 ENST00000429541 ENST00000399602 ENST00000354599 ENST00000528796 ENST00000599056 ENST00000601421 ENST00000599247 ENST00000601982 ENST00000355147 ENST00000429604 ENST00000418871 ENST00000434248 ENST00000221355 ENST00000598845 ENST00000593605 ENST00000588873 ENST00000356958 ENST00000392157 ENST00000292841 ENST00000355533 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF529
Gene Name:   zinc finger protein 529
Aliases:   KIAA1615
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000186020
Entrez:   57711
Chromosome Mapping:   19q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.890000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download