PrimePCR™ SYBR® Green Assay: MORF4L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055028

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MORF4L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MORF4L1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047768
Assay Design:   exonic
Chromosome Location:   15:79177424-79179615question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000379535 ENST00000426013 ENST00000331268 ENST00000559930 ENST00000558746 ENST00000558830 ENST00000559345 ENST00000558502 ENST00000559690 ENST00000559158 ENST00000560422 ENST00000559244 ENST00000559751 ENST00000410026 ENST00000360458 ENST00000372620 ENST00000441076 ENST00000434230 ENST00000418819 ENST00000442614 ENST00000422355 ENST00000433176 ENST00000422154 ENST00000451301 ENST00000423833

Gene Information

Description Not Available

Gene Symbol:   MORF4L1
Gene Name:   mortality factor 4 like 1
Aliases:   Eaf3, HsT17725, MEAF3, MGC10631, MORFRG15, MRG15, S863-6
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000185787
Entrez:   10933
Chromosome Mapping:   15q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.450000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download