PrimePCR™ SYBR® Green Assay: HIST1H2BL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054983

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIST1H2BL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIST1H2BL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047723
Assay Design:   exonic
Chromosome Location:   6:27775269-27775428question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000549491 ENST00000547561 ENST00000552971 ENST00000550101 ENST00000548739 ENST00000553240 ENST00000549836 ENST00000383219 ENST00000443138 ENST00000548637 ENST00000548137 ENST00000374708 ENST00000341947 ENST00000357486 ENST00000374714 ENST00000374713 ENST00000395197 ENST00000374712 ENST00000361917 ENST00000448717 ENST00000551758 ENST00000551536 ENST00000552556 ENST00000551786 ENST00000547883 ENST00000551572 ENST00000550998 ENST00000377401 ENST00000447349 ENST00000549885 ENST00000552765 ENST

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H2BL
Gene Name:   histone cluster 1, H2bl
Aliases:   H2B/c, H2BFC, dJ97D16.4
RefSeq:   NC_000006.11 NG_000009.2 NT_007592.15
Ensembl:   ENSG00000185130
Entrez:   8340
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.070000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download