PrimePCR™ SYBR® Green Assay: NT5C1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054971

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NT5C1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NT5C1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047711
Assay Design:   exonic
Chromosome Location:   2:18745152-18745297question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000594436 ENST00000597784 ENST00000263369 ENST00000597140 ENST00000532967 ENST00000304081 ENST00000359846 ENST00000418427 ENST00000600945

Gene Information

Cytosolic 5-prime nucleotidases such as NT5C1B catalyze production of adenosine which regulates diverse physiologic processes (Sala-Newby and Newby 2001 [PubMed 11690631]).[supplied by OMIM Mar 2008]

Gene Symbol:   NT5C1B
Gene Name:   5'-nucleotidase, cytosolic IB
Aliases:   AIRP, CN-IB, CN1B, MGC26640
RefSeq:   NC_000002.11 NT_015926.15
Ensembl:   ENSG00000185013
Entrez:   93034
Chromosome Mapping:   2p24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download