PrimePCR™ SYBR® Green Assay: BEX5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054937

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BEX5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BEX5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047677
Assay Design:   exonic
Chromosome Location:   X:101409017-101409161question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000444154 ENST00000378662 ENST00000234800 ENST00000407835 ENST00000404724 ENST00000437685 ENST00000263201 ENST00000300851 ENST00000340001 ENST00000453985 ENST00000370732 ENST00000333643 ENST00000543160 ENST00000361298 ENST00000372645 ENST00000372635 ENST00000372634 ENST00000299872 ENST00000594094 ENST00000602137

Gene Information

Description Not Available

Gene Symbol:   BEX5
Gene Name:   brain expressed, X-linked 5
Aliases:   MGC104434, MGC126446, NGFRAP1L1
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000184515
Entrez:   340542
Chromosome Mapping:   Xq22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.240000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download