PrimePCR™ SYBR® Green Assay: LENG9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control; Same primer pair as used in probe assay qHsaCEP0054818

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LENG9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LENG9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047558
Assay Design:   exonic
Chromosome Location:   19:54973866-54973977question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000453547 ENST00000266058 ENST00000343304 ENST00000462228 ENST00000393562 ENST00000393564 ENST00000369620 ENST00000439227 ENST00000440967 ENST00000272433 ENST00000393651 ENST00000357311 ENST00000489828 ENST00000474770 ENST00000448092 ENST00000547450 ENST00000462446 ENST00000304311 ENST00000416153 ENST00000369697 ENST00000315987 ENST00000302127 ENST00000452633 ENST00000333834 ENST00000503427 ENST00000502717 ENST00000594771 ENST00000593787 ENST00000595441 ENST00000600246 ENST00000601270 ENST

Gene Information

Description Not Available

Gene Symbol:   LENG9
Gene Name:   leukocyte receptor cluster (LRC) member 9
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000182909
Entrez:   94059
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.110000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download