PrimePCR™ SYBR® Green Assay: ADI1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054795

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ADI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADI1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047535
Assay Design:   exonic
Chromosome Location:   2:3502506-3502652question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000327435 ENST00000382093 ENST00000415131

Gene Information

This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. [provided by RefSeq May 2010]

Gene Symbol:   ADI1
Gene Name:   acireductone dioxygenase 1
Aliases:   APL1, ARD, FLJ10913, MTCBP1, Ni-ARD, SIPL
RefSeq:   NC_000002.11 NT_022221.13
Ensembl:   ENSG00000182551
Entrez:   55256
Chromosome Mapping:   2p25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.570000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download