PrimePCR™ SYBR® Green Assay: ZNF443, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054694

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF443, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF443, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047434
Assay Design:   exonic
Chromosome Location:   19:12551782-12551918question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000301215 ENST00000356020 ENST00000391809 ENST00000336334 ENST00000314797 ENST00000301547 ENST00000588883 ENST00000265165 ENST00000379951 ENST00000456128 ENST00000482155 ENST00000369915 ENST00000369912 ENST00000277165 ENST00000333936 ENST00000340893 ENST00000284548 ENST00000570156 ENST00000366706 ENST00000422127 ENST00000359599 ENST00000366707 ENST00000366709 ENST00000421798 ENST00000453825 ENST00000599466 ENST00000596987 ENST00000601851

Gene Information

Zinc finger proteins (ZNFs) bind DNA and through this binding regulate gene transcription. Most ZNFs contain conserved C2H2 motifs and are classified as Kruppel-type zinc fingers. For a general description of these proteins see ZNF91 (MIM 603971).[supplied by OMIM Jul 2002]

Gene Symbol:   ZNF443
Gene Name:   zinc finger protein 443
Aliases:   ZK1
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000180855
Entrez:   10224
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.500000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download