This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0054443
PrimePCR™ PreAmp for SYBR® Green Assay: CLEC14A, Human
PrimePCR™ Template for SYBR® Green Assay: CLEC14A, Human
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions such as cell adhesion cell-cell signalling glycoprotein turnover and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium it is considered to be a candidate for tumor vascular targeting. [provided by RefSeq Jan 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.