PrimePCR™ SYBR® Green Assay: AMIGO3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054410

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AMIGO3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AMIGO3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047149
Assay Design:   exonic
Chromosome Location:   3:49754685-49754788question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000294066 ENST00000320431 ENST00000535833 ENST00000480687 ENST00000297323 ENST00000297591 ENST00000437199 ENST00000558726 ENST00000359440 ENST00000297873 ENST00000394253 ENST00000373010 ENST00000373006 ENST00000373009 ENST00000297229 ENST00000409855 ENST00000409043 ENST00000409728 ENST00000409195 ENST00000409273 ENST00000409605 ENST00000409756 ENST00000420519 ENST00000295237 ENST00000325239 ENST00000319349 ENST00000393664 ENST00000545954 ENST00000545089 ENST00000591713 ENST00000538265 ENST

Gene Information

Description Not Available

Gene Symbol:   AMIGO3
Gene Name:   adhesion molecule with Ig-like domain 3
Aliases:   AMIGO-3, MGC120552
RefSeq:   NC_000003.11 NG_011603.1 NT_022517.18
Ensembl:   ENSG00000176020
Entrez:   386724
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 37.480000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download