PrimePCR™ SYBR® Green Assay: SPATA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054265

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047004
Assay Design:   exonic
Chromosome Location:   2:231861111-231861213question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000424440 ENST00000452881 ENST00000433428 ENST00000455816 ENST00000440792 ENST00000423134

Gene Information

Description Not Available

Gene Symbol:   SPATA3
Gene Name:   spermatogenesis associated 3
Aliases:   TSARG1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000173699
Entrez:   130560
UniGene:   Hs.121021
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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