PrimePCR™ SYBR® Green Assay: ZNF449, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054232

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF449, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF449, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046971
Assay Design:   exonic
Chromosome Location:   X:134481262-134481395question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000370761 ENST00000339249 ENST00000370760 ENST00000596735 ENST00000593526 ENST00000594498

Gene Information

This gene encodes a nuclear protein that likely functions as a transcription factor. The protein includes an N-terminal SCAN domain and seven C2H2-type zinc finger motifs. [provided by RefSeq May 2010]

Gene Symbol:   ZNF449
Gene Name:   zinc finger protein 449
Aliases:   FLJ23614, ZSCAN19
RefSeq:   NC_000023.10 NG_021220.1 NT_011786.16
Ensembl:   ENSG00000173275
Entrez:   203523
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 37.080000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download