PrimePCR™ SYBR® Green Assay: TPSAB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054146

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TPSAB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TPSAB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046885
Assay Design:   exonic
Chromosome Location:   16:1292181-1292278question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000338844 ENST00000461509 ENST00000561736 ENST00000397534 ENST00000369569 ENST00000256658 ENST00000343629 ENST00000430512

Gene Information

Tryptases comprise a family of trypsin-like serine proteases the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation such that the alleles were once thought to represent two separate genes alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq Jul 2008]

Gene Symbol:   TPSAB1
Gene Name:   tryptase alpha/beta 1
Aliases:   MGC104064, TPS1, TPS2, TPSB1
RefSeq:   NC_000016.9 NG_005083.1 NT_010393.16
Ensembl:   ENSG00000172236
Entrez:   7177
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.030000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download