PrimePCR™ SYBR® Green Assay: KLF11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054130

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046869
Assay Design:   exonic
Chromosome Location:   2:10186407-10186512question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000401510 ENST00000305883 ENST00000448523 ENST00000440320 ENST00000540845 ENST00000535335

Gene Information

The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Apr 2010]

Gene Symbol:   KLF11
Gene Name:   Kruppel-like factor 11
Aliases:   FKLF, FKLF1, MODY7, TIEG2, Tieg3
RefSeq:   NC_000002.11 NT_005334.16 NG_017199.1
Ensembl:   ENSG00000172059
Entrez:   8462
UniGene:   Hs.12229
Chromosome Mapping:   2p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.830000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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