PrimePCR™ SYBR® Green Assay: ATPAF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054120

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046859
Assay Design:   exonic
Chromosome Location:   17:17929635-17931556question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000585101 ENST00000474627 ENST00000444058

Gene Information

This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described but its biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   ATPAF2
Gene Name:   ATP synthase mitochondrial F1 complex assembly factor 2
Aliases:   ATP12, ATP12p, MGC29736
RefSeq:   NC_000017.10 NG_012824.1 NT_010718.16
Ensembl:   ENSG00000171953
Entrez:   91647
UniGene:   Hs.528889
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.410000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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